The potential use of chicken blastodermal cells of Oravka breed

Abstract

This study provides information about the method of isolation as well as evaluation of morfology and viability of chicken blastodermal cells (BCs) of Oravka breed. Oravka breed belongs to a critical endangered breed under the conditions of the Slovak Republic. Due to this fact, it is necessary to preserve genetic material of Oravka breed. Frozen blastodermal cells are the potential tool for long-term storage. Chicken blastodermal cells were derived from X stage of embryos (blastoderm stage) using filter paper ring method. To detect blastodermal cells viability specific nuclear fluorochrome Yo-Pro-1 in combination with propidium iodide and DAPI were used. Under the light microscope the isolated blastodermal cells were seen as a large round cells with diameter from 13 to 18 μm and acentric nucleus of 9 μm in diameter. Fluorescent analysis revealed only viable, DAPI-positive cells in the samples. Totally, 38000 blastodermal cells of Oravka breed from each blastoderm were obtained.