Level of Chromosomal Aneuploidy Detected from Peripheral Blood and Bone Marrow in Transgenic Rabbits
Keywords:
transgene, rabbit, hFVIII, bone marrow lymphocytes, peripheral blood lymphocytes, chromosomal aneuploidyAbstract
Our study aimed at comparing the chromosomal aneuploidy rate between transgenic and non-transgenic rabbits with respect to the source of cells: bone marrow vs. Peripheral blood culture. Samples were obtained from New Zealand White transgenic (carrying human factor VIII gene) and non-transgenic rabbits. C-metaphase plates were evaluated from bone marrow lymphocytes and peripheral blood lymphocyte samples synchronized by the addition of 0.25 μg/ ml colcemide. No significant difference in the chromosomal aneuploidy between transgenic (34.45% from blood vs. 62.8% from bone marrow) and non-transgenic (37.78% from blood vs. 56.1% from bone marrow) rabbits was observed. Higher chromosomal aneuploidy rate was detected from samples of bone marrow than from the peripheral blood cells. In conclusion: hFVIII transgene does not influence aneuploidy rate in transgenic rabbits.
