EFFECT OF IN OVO ARGININE INJECTION ON GASTROINTESTINAL AND HAEMATO-BIOCHEMICAL INDICATORS OF FUNAAB-ALPHA CHICKENS
Keywords:
in ovo injection, performance, health status, duodenal villi, gastro-intestinalAbstract
This study investigated the effects of in ovo administration of arginine on duodenal villi development, weight gain and haemato-biochemical indices of FUNAAB-Alpha chickens. A total of 528 hatching eggs of FUNAAB-Alpha chickens were used in the experiment. The hatching eggs were balanced for weight, fumigated and, thereafter, set in the incubator. On 14th day of incubation, 378 eggs were confirmed fertile and assigned into 3 treatments (0, 11 and 22 mg of arginine/egg) each consisting of 126 eggs, replicated 14 times (9 eggs per replicate). At 18th day of embryonic age, in ovo administration of arginine was carried out and at 21st day of incubation, resulting chicks were balanced for weight on treatments basis, assigned to replicates and they were assessed for post-hatch performance. Data on gastro-intestinal development and haemato-biochemical indices were subjected to Completely Randomized Design. In the results, influence of in ovo injection of arginine (11 and 22 mg per egg) on duodenal histology of FUNAAB-Alpha chickens (at 14 days of age) resulted in marked improvement in the duodenal villi, as against that observed in birds under the control (without in ovo injection of arginine). Also, in ovo administration of arginine did not pose any deleterious effects on the haemato-biochemical indices of FUNAAB-Alpha chickens (at 4 weeks of age), as the values recorded were within normal ranges for healthy chickens. In conclusion, for enhanced performance in terms of duodenal villi, gastrointestinal development and haemato-biochemical indices of FUNAAB-Alpha chickens the in ovo injection of arginine (up to 22 mg/egg) is suitable without negative implications on the health status of the birds at 4 weeks of age.
References
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<br>Al-Asadi, A. N. O. (2013). Effect of early feeding (in ovo injection) amino acids on hatchability, some productive and physiology traits of broiler. Journal of Applied Science and Technology, 8, 6−13. http://dx.doi.org/10.12816/0000224
<br>Al-Daraji, H. J., Al-Mashadani, A. A., Al-Hayani, W. K., Al-Hassani, A. S. & Mirza, H. A. (2012). Effect of in-ovo injection with L-arginine on productive and physiological traits of Japanese quail. South African Journal of Animal Science, 42(2), 139−145. http://dx.doi.org/10.4314/sajas.v42i2.6
<br>Awachat, V. B., Elangovan, A. V., Jose, N., David, C. G., Ghosh, J., Bhanja, S. K. & Majumdar, S. (2017). Influence of perinatal amino acid supplementation on hatchability, gastro-intestinal tract development and growth performance of broiler chicks. Tropical Animal and Poultry Science, 1, 28−41.
<br>Bhanja, S. K, Mandal, A. B., Agarwal, S. K. & Majumdar, S. (2012). Modulation of post-hatch-growth and immunocompetence through in ovo injection of limiting amino acids in broiler chickens. Indian Journal of Animal Sciences, 82, 993−998.
<br>Bhanja, S. K., Mandal, A. B. & Johri, T. S. (2004). Standardization of injection sites, needle length, embryonic age and concentration of amino acids for in ovo injected in broiler breeder eggs. Indian Journal of Poultry Science, 39, 105−111.
<br>Careghi, C., Tona, K., Onagbesan, O., Buyse, J., Decuypere E., & Bruggeman, V. (2005). The effects of the spread of hatch and interaction with delayed feed access after hatch on broiler performance until seven days of age. Poultry Science, 84, 1314−1320. https://doi.org/10.1093/ps/84.8.1314
<br>D'Amato, J. L. & Humphrey, B. D. (2010). Dietary arginine levels alter markers of arginine utilization in peripheral blood mononuclear cells and thymocytes in young broiler chicks. Poultry Science, 89, 938−947. http://dx.doi.org/10.3382/ps.2009-00611
<br>Deng, K., Wong, C. W. & Nolan, C. W. (2005). Long-term effects of early life L-arginine supplementation on growth performance, lymphoid organs and immune responses in Leghorn type chickens. British Poultry Science, 46, 318−324. http://dx.doi.org/10.1080/00071660500127449
<br>Dibner, J. J., Knight, C. D., Kitchell, M. L., Atwell, C.A., Downs, A. C., & Ivey, F. J. (1998). Early feeding and development of the immune system in neonatal poultry. Journal of Applied Poultry Research, 7, 425−436. http://dx.doi.org/10.1093/japr/7.4.425
<br>Dror, Y., Nir, I. & Nitsan, Z. (1997). The relative growth of internal organs in light and heavy breeds. British Poultry Science, 18, 493−496. https://doi.org/10.1080/00071667708416389
<br>Foye, O. T., Ferket, P. R. & Uni, Z. (2007). The effects of in ovo feeding arginine, β-hydroxy-β-methyl-butyrate, and protein on jejunal digestive and absorptive activity in embryonic and neonatal turkey poults. Poultry Science, 86(11), 2343−2349. https://doi.org/10.3382/ps.2007-00110
<br>Google Maps. 2019. Federal University of Agriculture. Retrieved from https://earth.google.com/web/@7.22330744,3.44033719,137.84884575a,1046.69760578d,35y,100.57030218h,44.99999706t,-0r/data=Cm4abBJmCiUweDEwM.
<br>Ikhimioya, I., Arijeniwa, A., Oteku, I. T. & Ahmed, A. (2000). Preliminary investigation on the haematology of the Nigerian indigenous chicken. Proceedings of the 5th Annual Conference of Animal Science Association of Nigeria. Port Harcourt, Nigeria, 10−12.
<br>Jain, N. C. (1986). Schalm's Veterinary Hematology. 4th ed. Lea and Febiger, 600. Washington square, Philadelphia, USA.
<br>Khajali, F. & Wideman, R. F. (2010). Dietary arginine: metabolic, environmental, immunological and physiological interrelationships. World Poultry Science Journal, 66(4), 751−766. http://dx.doi.org/10.1017/S0043933910000711
<br>Moore, D. T., Ferket, P. R. & Mozdziak, P. E. (2005). The effect of early nutrition on satellite cell dynamics in the young turkey. Poultry Science, 84, 748−756. http://dx.doi.org/10.1093/ps/84.5.748
<br>Mitruka, B. M. & Rawnsley, H. M. (1977). Clinical Biochemical and Haematological Reference Value in Normal Experimental Animals. Masson Publication, New York, USA, ISBN-13: 9780893520069, 21−64.
<br>Moosavinasab, F., Ghalehkand, J. G., Ghanbari, O. & Hassanpour, S. (2015). In ovo injection of IGF1 improves intestinal enzyme activity in broilers. European Poultry Science, 79, 1−12. DOI: 10.1399/eps.2015.94
<br>Muhammad, W. B., Aqeela, R. Aamir, N., Shuyan, T., Muhammad, W. G., Guanghui, L., Mei, X. & Lilong, A. (2019). Importance of arginine as immune regulator in Animal Nutrition. International Journal of Veterinary Sciences Research, 5(1), 1−10. DOI: 10.18488/journal.110.2019.51.1.10
<br>Nayak, N., Rajini, R. A., Ezhilvalavan, S., Sahu, A. R. & Kirubaharan, J. J. (2016). Influence of in ovo arginine feeding on post-hatch growth performance and economics of broilers. Journal of Animal Research, 6(4), 585−591. http://dx.doi.org/10.5958/2277-940X.2016.00067.X
<br>Noy, Y. & Sklan, D. (1999). Different types of early feeding and performance in chicks and poults. Journal of Applied Poultry Research, 8, 16−24. http://dx.doi.org/10.1093/japr/8.1.16
<br>Noy, Y. & Uni, Z. 2010. Early nutritional strategies. World Poultry Science Journal, 66, 639−646. http://dx.doi.org/10.1017/S0043933910000620
<br>Nworgu, F. C., Ogungbenro, S. A. & Solesi, K. S. (2007). Performance and some blood chemistry indices of broiler chicken served fluted pumpkin (Telfaria occidentalis) leaves extract. American Eurasian Journal of Agriculture and Environmental Science, 2(1), 90−98.
<br>Odutayo, O. J., Sogunle, O. M., Adeyemi, O. A. & Sonibare, A. O. (2020). Influence of in ovo arginine feeding on hatching traits and post-hatch performance of FUNAAB-Alpha chickens in two housing types. Tropical Animal Health and Production, 52(5), 2349−2357. http://doi.org/10.1007/s11250-020-02258-x.
<br>Pegg, A. E. & McCann, P. P. (1982). Polyamine metabolism and function. American Journal of Physiology, 243: C212. https://doi.org/10.1152/ajpcell.1982.243.5.C212
<br>Riddell, C. (2011). Comparative anatomy, histology and physiology of the chicken. Department of pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, S7N0W0.pg37. http://cal.vet.upenn.edu/projects/poultry/Syllabus/page37_44.htm.
<br>Ross, J. G., Christie, G., Halliday, W. G. & Jones, R. M. (1978). Hematological and blood chemistry comparison values for clinical pathology in poultry. The Veterinary Record, 102(2), 29−31. https://doi.org/10.1136/vr.102.2.29
<br>Sahr, W. B. K., Odutayo, O. J., Sogunle, O. M., Ayo-Ajasa, O. Y., Fafiolu, A. O. & Fatunmbi, F. A. (2020). Effects of in ovo injection of inorganic salts of Zn, Cu and Mn on hatching traits and post-hatch performance of broiler chickens in the tropics. Nigerian Journal of Animal Science, 22 (1), 113−125.
<br>Shamoto, K. & Yamauchi, K. (2000). Recovery responses of chick intestinal villus morphology to different refeeding procedures. Poultry Science, 79, 718−723. https://doi.org/10.1093/ps/79.5.718
<br>Silva, L. M. G. S., Murakami, A. E., Fernandes, J. I. M., Dalla Rosa, D. & Urgnani, J. F. (2012). Effects of dietary arginine supplementation on broiler breeder egg production and hatchability. British Poultry Science, 14, 233−304. http://dx.doi.org/10.1590/S1516-635X2012000400006
<br>Sogunle, O. M., Elangovan, A. V., David, C. G., Gosh, J. & Awachat, V. B. (2018). Response of broiler chickens to in ovo administration of inorganic salts of Zinc, Selenium and Copper or their combination. Slovak Journal of Animal Science, 51(1), 8−19.
<br>Sogunle, O. M., Olutayo, A. J., Odutayo, O. J., Oso, O. A., Sobukola, O. P., Safiyu, K. K. & Elangovan, A. V. (2019). Effects of in ovo injection of amino acids on hatching performance, cell-mediated immunity and blood profile of FUNAAB Alpha broiler chickens. Nigerian Journal of Animal Production, 46(4), 59−72. https://doi.org/10.51791/njap.v46i4.998
<br>Tamir, H. & Ratner, S. (1963). A study of ornithine, citrulline and arginine synthesis in growing chicks. Archives of Biochemistry and Biophysics, 102(2), 259−269. https://doi.org/10.1016/0003-9861(63)90179-6
<br>Tona, K. F., Barnelis, B., De Kettelaere, V., Bruggerman, V., Moraes, B. M., Vuyse, J., Onagbesan, O. & Decuypere, E. (2003). Effects of egg storage time on spread of hatch, chick quality and chick juvenile growth. Poultry Science, 82, 736−741. https://doi.org/10.1093/ps/82.5.736
<br>Uni, Z. & Ferket, P. R. (2004). Methods for early nutrition and their potential. World Poultry Science Journal, 60, 101−111. https://doi.org/10.1079/WPS20038</div>
<br>Alabi, A. J., Ng'ambi, J. W., Mbajiorgu, E. F., Norris, D. & Mabelebele. M. (2015). Growth and haematological response of indigenous Venda chickens aged 8 to 13 weeks to varying dietary lysine to energy ratios. Journal of Animal Physiology Animal Nutrition, 99, 436−441. https://doi.org/10.1111/jpn.12277
<br>Al-Asadi, A. N. O. (2013). Effect of early feeding (in ovo injection) amino acids on hatchability, some productive and physiology traits of broiler. Journal of Applied Science and Technology, 8, 6−13. http://dx.doi.org/10.12816/0000224
<br>Al-Daraji, H. J., Al-Mashadani, A. A., Al-Hayani, W. K., Al-Hassani, A. S. & Mirza, H. A. (2012). Effect of in-ovo injection with L-arginine on productive and physiological traits of Japanese quail. South African Journal of Animal Science, 42(2), 139−145. http://dx.doi.org/10.4314/sajas.v42i2.6
<br>Awachat, V. B., Elangovan, A. V., Jose, N., David, C. G., Ghosh, J., Bhanja, S. K. & Majumdar, S. (2017). Influence of perinatal amino acid supplementation on hatchability, gastro-intestinal tract development and growth performance of broiler chicks. Tropical Animal and Poultry Science, 1, 28−41.
<br>Bhanja, S. K, Mandal, A. B., Agarwal, S. K. & Majumdar, S. (2012). Modulation of post-hatch-growth and immunocompetence through in ovo injection of limiting amino acids in broiler chickens. Indian Journal of Animal Sciences, 82, 993−998.
<br>Bhanja, S. K., Mandal, A. B. & Johri, T. S. (2004). Standardization of injection sites, needle length, embryonic age and concentration of amino acids for in ovo injected in broiler breeder eggs. Indian Journal of Poultry Science, 39, 105−111.
<br>Careghi, C., Tona, K., Onagbesan, O., Buyse, J., Decuypere E., & Bruggeman, V. (2005). The effects of the spread of hatch and interaction with delayed feed access after hatch on broiler performance until seven days of age. Poultry Science, 84, 1314−1320. https://doi.org/10.1093/ps/84.8.1314
<br>D'Amato, J. L. & Humphrey, B. D. (2010). Dietary arginine levels alter markers of arginine utilization in peripheral blood mononuclear cells and thymocytes in young broiler chicks. Poultry Science, 89, 938−947. http://dx.doi.org/10.3382/ps.2009-00611
<br>Deng, K., Wong, C. W. & Nolan, C. W. (2005). Long-term effects of early life L-arginine supplementation on growth performance, lymphoid organs and immune responses in Leghorn type chickens. British Poultry Science, 46, 318−324. http://dx.doi.org/10.1080/00071660500127449
<br>Dibner, J. J., Knight, C. D., Kitchell, M. L., Atwell, C.A., Downs, A. C., & Ivey, F. J. (1998). Early feeding and development of the immune system in neonatal poultry. Journal of Applied Poultry Research, 7, 425−436. http://dx.doi.org/10.1093/japr/7.4.425
<br>Dror, Y., Nir, I. & Nitsan, Z. (1997). The relative growth of internal organs in light and heavy breeds. British Poultry Science, 18, 493−496. https://doi.org/10.1080/00071667708416389
<br>Foye, O. T., Ferket, P. R. & Uni, Z. (2007). The effects of in ovo feeding arginine, β-hydroxy-β-methyl-butyrate, and protein on jejunal digestive and absorptive activity in embryonic and neonatal turkey poults. Poultry Science, 86(11), 2343−2349. https://doi.org/10.3382/ps.2007-00110
<br>Google Maps. 2019. Federal University of Agriculture. Retrieved from https://earth.google.com/web/@7.22330744,3.44033719,137.84884575a,1046.69760578d,35y,100.57030218h,44.99999706t,-0r/data=Cm4abBJmCiUweDEwM.
<br>Ikhimioya, I., Arijeniwa, A., Oteku, I. T. & Ahmed, A. (2000). Preliminary investigation on the haematology of the Nigerian indigenous chicken. Proceedings of the 5th Annual Conference of Animal Science Association of Nigeria. Port Harcourt, Nigeria, 10−12.
<br>Jain, N. C. (1986). Schalm's Veterinary Hematology. 4th ed. Lea and Febiger, 600. Washington square, Philadelphia, USA.
<br>Khajali, F. & Wideman, R. F. (2010). Dietary arginine: metabolic, environmental, immunological and physiological interrelationships. World Poultry Science Journal, 66(4), 751−766. http://dx.doi.org/10.1017/S0043933910000711
<br>Moore, D. T., Ferket, P. R. & Mozdziak, P. E. (2005). The effect of early nutrition on satellite cell dynamics in the young turkey. Poultry Science, 84, 748−756. http://dx.doi.org/10.1093/ps/84.5.748
<br>Mitruka, B. M. & Rawnsley, H. M. (1977). Clinical Biochemical and Haematological Reference Value in Normal Experimental Animals. Masson Publication, New York, USA, ISBN-13: 9780893520069, 21−64.
<br>Moosavinasab, F., Ghalehkand, J. G., Ghanbari, O. & Hassanpour, S. (2015). In ovo injection of IGF1 improves intestinal enzyme activity in broilers. European Poultry Science, 79, 1−12. DOI: 10.1399/eps.2015.94
<br>Muhammad, W. B., Aqeela, R. Aamir, N., Shuyan, T., Muhammad, W. G., Guanghui, L., Mei, X. & Lilong, A. (2019). Importance of arginine as immune regulator in Animal Nutrition. International Journal of Veterinary Sciences Research, 5(1), 1−10. DOI: 10.18488/journal.110.2019.51.1.10
<br>Nayak, N., Rajini, R. A., Ezhilvalavan, S., Sahu, A. R. & Kirubaharan, J. J. (2016). Influence of in ovo arginine feeding on post-hatch growth performance and economics of broilers. Journal of Animal Research, 6(4), 585−591. http://dx.doi.org/10.5958/2277-940X.2016.00067.X
<br>Noy, Y. & Sklan, D. (1999). Different types of early feeding and performance in chicks and poults. Journal of Applied Poultry Research, 8, 16−24. http://dx.doi.org/10.1093/japr/8.1.16
<br>Noy, Y. & Uni, Z. 2010. Early nutritional strategies. World Poultry Science Journal, 66, 639−646. http://dx.doi.org/10.1017/S0043933910000620
<br>Nworgu, F. C., Ogungbenro, S. A. & Solesi, K. S. (2007). Performance and some blood chemistry indices of broiler chicken served fluted pumpkin (Telfaria occidentalis) leaves extract. American Eurasian Journal of Agriculture and Environmental Science, 2(1), 90−98.
<br>Odutayo, O. J., Sogunle, O. M., Adeyemi, O. A. & Sonibare, A. O. (2020). Influence of in ovo arginine feeding on hatching traits and post-hatch performance of FUNAAB-Alpha chickens in two housing types. Tropical Animal Health and Production, 52(5), 2349−2357. http://doi.org/10.1007/s11250-020-02258-x.
<br>Pegg, A. E. & McCann, P. P. (1982). Polyamine metabolism and function. American Journal of Physiology, 243: C212. https://doi.org/10.1152/ajpcell.1982.243.5.C212
<br>Riddell, C. (2011). Comparative anatomy, histology and physiology of the chicken. Department of pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, S7N0W0.pg37. http://cal.vet.upenn.edu/projects/poultry/Syllabus/page37_44.htm.
<br>Ross, J. G., Christie, G., Halliday, W. G. & Jones, R. M. (1978). Hematological and blood chemistry comparison values for clinical pathology in poultry. The Veterinary Record, 102(2), 29−31. https://doi.org/10.1136/vr.102.2.29
<br>Sahr, W. B. K., Odutayo, O. J., Sogunle, O. M., Ayo-Ajasa, O. Y., Fafiolu, A. O. & Fatunmbi, F. A. (2020). Effects of in ovo injection of inorganic salts of Zn, Cu and Mn on hatching traits and post-hatch performance of broiler chickens in the tropics. Nigerian Journal of Animal Science, 22 (1), 113−125.
<br>Shamoto, K. & Yamauchi, K. (2000). Recovery responses of chick intestinal villus morphology to different refeeding procedures. Poultry Science, 79, 718−723. https://doi.org/10.1093/ps/79.5.718
<br>Silva, L. M. G. S., Murakami, A. E., Fernandes, J. I. M., Dalla Rosa, D. & Urgnani, J. F. (2012). Effects of dietary arginine supplementation on broiler breeder egg production and hatchability. British Poultry Science, 14, 233−304. http://dx.doi.org/10.1590/S1516-635X2012000400006
<br>Sogunle, O. M., Elangovan, A. V., David, C. G., Gosh, J. & Awachat, V. B. (2018). Response of broiler chickens to in ovo administration of inorganic salts of Zinc, Selenium and Copper or their combination. Slovak Journal of Animal Science, 51(1), 8−19.
<br>Sogunle, O. M., Olutayo, A. J., Odutayo, O. J., Oso, O. A., Sobukola, O. P., Safiyu, K. K. & Elangovan, A. V. (2019). Effects of in ovo injection of amino acids on hatching performance, cell-mediated immunity and blood profile of FUNAAB Alpha broiler chickens. Nigerian Journal of Animal Production, 46(4), 59−72. https://doi.org/10.51791/njap.v46i4.998
<br>Tamir, H. & Ratner, S. (1963). A study of ornithine, citrulline and arginine synthesis in growing chicks. Archives of Biochemistry and Biophysics, 102(2), 259−269. https://doi.org/10.1016/0003-9861(63)90179-6
<br>Tona, K. F., Barnelis, B., De Kettelaere, V., Bruggerman, V., Moraes, B. M., Vuyse, J., Onagbesan, O. & Decuypere, E. (2003). Effects of egg storage time on spread of hatch, chick quality and chick juvenile growth. Poultry Science, 82, 736−741. https://doi.org/10.1093/ps/82.5.736
<br>Uni, Z. & Ferket, P. R. (2004). Methods for early nutrition and their potential. World Poultry Science Journal, 60, 101−111. https://doi.org/10.1079/WPS20038</div>
