BLOOD PROFILE AND PHYSIOLOGICAL RESPONSE OF BROILER CHICKENS TO SWEET ORANGE PEEL SUPPLEMENTED WATER IN A HOT HUMID ENVIRONMENT
DOI:
https://doi.org/10.36547/sjas.774Keywords:
Haematology, Rectal temperature, Respiratory rate, Serum biochemistry, Sweet citrus peel, Water supplementationAbstract
This study investigated the effect of sweet orange peel powder (SOPP) supplemented water on the rectal temperature (RT), respiratory rate (RR) and blood profile of broiler chickens raised in a hot humid environment. Day-old Ross broiler chickens (n = 96) were randomly allotted into four treatment groups: Control – without SOPP, 2 g SOPP, 4 g SOPP and 6 g SOPP per litre of water. Three replications of eight birds each per treatment in a completely randomized design were performed. Rectal temperatures were comparable (p > 0.05) among the treatments across the weeks except the third week. Birds on 6 g SOPP had a significantly (p < 0.05) higher value (41.32 °C) compared with birds in the control (40.90 °C) and 4 g SOPP (41.00 °C). Respiratory rate was not significantly different (p > 0.05) among treatments across the weeks. Significant (p < 0.05) variations were observed in the red blood cell (RBC) and mean corpuscular haemoglobin (MCH) values at week 4. However, at week 7, heterophil (H), lymphocyte (L), heterophil:lymphocyte ratio (H/L) and RBC were significantly (p < 0.05) different among the treatments. The H/L values were 0.42, 0.40, 0.38 and 0.36 for Control, 2 g SOPP, 4 g SOPP and 6 g SOPP, respectively. Serum biochemical indices examined were comparable among the treatment groups across the weeks. Inclusion of SOPP into drinking water of broiler birds did not adversely affect the birds as observed in the RT, RR and blood profile. Rather it helps the birds in coping with the environmental stress, as observed in the reduced H/L.
References
Abbasi, H., Seidavi, A. R., Liu, W. & Asadpour, L. (2015) Investigation on the effect of different levels of dried sweet orange (Citrus sinensis) pulp on performance, carcass characteristics and physiologicaland biochemical parameters in broiler chicken. Saudi Journal of Biological Sciences, 22(2), 139−143.
Abdulameer, Y. S. (2018). The effects of dietary vitamin C and Citrus Sinensis peel on growth, hematological characteristics, immune competence, and carcass characteristics in broilers exposed to heat stress. Iraqi Journal of Veterinary Sciences, 32(2), 253−260.
Amaga, A. O., Oluremi, O. I. A., Tuleun C. D. & Kaankuka F. G. (2019). Haematology and Serum Biochemical Constituents of Finisher Broiler Chickens Fed Diets Containing Water Soaked Sweet Orange (Citrus sinensis) Peel Meal at Varying Durations. American Journal of Experimental Agriculture, 41(3), 1−8.
Azizi, M., Seidavi, A. R., Ragni, M., Laudadio, V. & Tufarelli, V. (2018). Practical applications of agricultural wastes in poultry feeding in Mediterranean and Middle East regions. Part 1: citrus, grape, pomegranate and apple wastes. World's Poultry Science Journal, 74(3), 1−10. DOI: 10.1017/S0043933918000478
Behera, D. P., Sethi, A. P. S., Singh, C., Singh, U., & Wadhwa, M. (2019). Effect of citrus waste on blood parameters of broiler birds with and without cocktail of enzymes. Veterinary World, 12(4), 483−488. DOI: 10.14202/vetworld.2019.483-488
Bounous, D. I. & Stedman, N. L. (2000). Normal avian hematology: chicken and turkey. In: Feldman, B. F., Zinkl, J. G., Jain, N. C., editors. Schalm's Veterinary Hematology. New York: Wiley, 1147-1154.
Cahaner, A., Ajuh, J. A., Siegmund-Schultze, M., Azoulay, Y., Druyan S. & Valle Zarate, A. (2008). Effects of the genetically reduced feather coverage in naked neck and featherless broilers on their performance under hot conditions. Poultry Science, 87(12), 2517−2527.
Dinu, C., Pârvu, M., Andronie, I., Avram, N., Cucă, D., & Radoi, I. E. (2007). Interaction between the diet, temperature and some blood components in Roso layers. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies, 64, 138−142.
Hindi, A., Elagib, H. A. A., & Elzubeir, E. A. (2012). The humoral immune response of heat stressed broiler chicks fed different levels of energy and methionine. International Journal of Poultry Science, 11(6), 400−404. DOI: 10.3923/ijps.2012.400.404
Iskender, H., Yenice, G., Dokumacioglu, E., Kaynar, O., Hayirli, A. & Kaya, A. (2016). The effects of dietary flavonoid supplementation on the antioxidant status of laying hens. Brazilian Journal of Poultry Science, 18(4), 663−668.
Iyaode, I. I., Ibrahim, H. O., Uwade, F. & Shittu, M. W. (2020). Haematology and serum biochemistry of broiler strains (Cobbs and Arbor-acre) fed ginger (Zingiber officinale). GSC Biological and Pharmaceutical Sciences, 11(2), 320−326. https://doi.org/10.30574/gscbps.2020.11.2.0145
Jain, N. C. (1986). Scanning electron micrograph of blood cells. In: Schalm's Veterinary Haematology. 4th ed. Lea & Febiger. Philadelphia 4, 63−70.
Janero, D. R. (1990). Malondialdehyde and thiobarbituric acid reactivity as diagnositic indexes of lipid peroxidation and peroxidative tissue injury. Free Radical Biology and Medicine, 9(6), 515−540. DOI: 10.1016/0891-5849(90)90131
Kohn, R. A. & Allen, M. S. (1995). Enrichment of proteolysis activity relative to nitrogen in preparations from the rumen for in vitro studies. Animal Feed Science and Technology, 52(1-2), 1−4.
Kongpechr, S., Sukon, P. & Sohsuebngarm, D. (2020). The study of hematology in commercial broiler chickens of Cobb 500, Ross 308, and Arbor Acres Plus. Journal of Mahanakorn Veterinary Medcine, 15(2), 209−221.
Majekodunmi, B. C., Logunleko, M. O., Adekunle, E. O., Abioja, M. O., Akinjute, F. O., Owolabi, T. O., & Daramola, J. O. (2021). Evaluation of sweet citrus peel supplements in water on performance and ileal microbial count of broiler chickens. Tropical Animal Health and Production, 53, 405. https://doi.org/10.1007/s11250-021-02858-1
Manthey, J. A. (2004). Fractionation of orange peel phenols in ultrafiltered molasses and mass balance studies of their antioxidant levels. Journal of Agricultural and Food Chemistry, 52(25), 7586−7592.
Marai, I. F. M., Ayyat, M. S., & Abd El-Monem, U. M. (2001). Growth performance and reproductive traits at first parity of New Zealand White female rabbits as affected by heat stress and its alleviation under Egyptian conditions. Tropical Animal Health and Production, 33, 1−12.
Maya-Soriano, M. J. (2012). Heat stress and antioxidant agents: Effects on gamete development, (p. 120). PhD. dissertation at Animal medicine and Anatomy, Universitat Autonoma de Barcelona, Barcelona, Spain.
Medeiros, C. M. (2001). Ajuste de modelos e determinação de índicetérmicoambiental de produtividade para frangos de corte. D.S. dissertation, Universidade Federal de Viçosa.
Mitruka, H. M. & Rawnsley, S. K. (1997). Chemical, Biochemical and Haematological Reference Values in Normal Experimental Animals. Masson Publishing, New York, USA, 287.
Nascimento, S. T., da Silva, I. J. O., Mourao, G. B. & Castro, A. C. (2012). Bands of respiratory rate and cloacal temperature of four different broiler chicken strains. Revista Brasileira de Zootecnia, 41(5), 1318−1324.
National Research Council. (1994). Nutrient Requirements of Poultry: Ninth Revised Edition. The National Academies Press, Washington, D.C.
Orayaga, K. T., Oluremi, O. I. A., & Adenkola, A. I. (2016). Effect of Water Soaking of Sweet Orange (Citrus sinensis) Fruit Peels on Haematology, Carcass Yield and Internal Organs of Finisher Broiler Chickens. Journal of Animal Health and Production, 4(3), 65−71.
Peters, T., Biamote, G. T. & Doumas, B. T. (1982). Total Protein in Serum, Urine, and Cerebrospinal Fluid, Albumin, in Serum. In: Faulkner, W. R. and Meites, S., Eds., Selected Methods of Clinical Chemistry, American Association for Clinical Chemistry, Volume 9, Washington D.C.
Pourhossein, Z., Qotbi, A. A. A., Seidavi, A. R. Laudadio, V., Centoducati, G. & Tufarelli, V. (2015). Effect of different levels of dietary sweet orange (Citrus sinensis) peel extract on humoral immune system responses in broiler chickens. Animal Science Journal, 86, 105−110.
Rej, R. & Holder, H. (1983). Aspartate transaminase. In: Methods of Enzymatic Analysis. 3rd ed (H. U. Bergmeyer, J. Bergmeyer and M.Grassl, Eds). WeinheinVerlag- Chemie, 3, 416−433.
Renaudeau, D., Collin, A., Yahav, S., de Basilio, V., Gourdine, J. L. & Collier, R. J. (2012). Adaptation to hot climate and strategies to alleviate heat stress in livestock production. Animal, 6, 707−728.
SAS (1999). SAS/STAT User's Guide. Version 8 for Windows. SAS Institute Inc., SAS Campus Drive, Cary, North Carolina, USA.
Schalm, O. W., Jain, N. C. & Carrol, E. G. (1975). Veterinary Haematology, 3rd ed. Leo and Febiger, Philadelphia, 140−152.
Seidavi, A., Ebrahimi, A., AlawQotbi, A. A. & Gómez Nieto, J. A. (2015). Effect of different levels of Citrus sinensis peel extract on broiler performance, blood parameters, thyroid gland activity and bone ash. Indian Journal of Animal Sciences, 85(11), 1225−1228.
Soleimani, A. F., Kasim, A., Alimon, A. R. & Zulkifli, I. (2008). Durability of induced heat tolerance by short term heat challenge at broilers marketing age. Pakistan Journal of Biologica Sciences, 11, 2163−2166.
Spiers, D. E., Spain, J. N., Sampson, J. D. & Rhoads, R. P. (2004). Use of physiological parameters to predict milk yield and feed intake in heat stressed dairy cows. Journal of Thermal Biology, 29(7-8), 759−764.
Vlaicu, P. A, Turcu, R. P., Mironeasa, S. & Panaite, T. D. (2020). Meat quality of breast from broilers fed a diet supplemented with orange and red grapefruit dried peel. Scientific Papers. Series D. Animal Science, 63(1), 161−169.
Yalcin, S., Ozkan, S., Cabuk, M., Buyse, J., Decuypere, E. & Siegel, P. B. (2005). Pre- and postnatal conditioning induced thermo tolerance on body weight, physiological responses and relative asymmetry of broilers originating from young and old breeder flocks. Poultry Science, 84, 967−976.
Yang, X. Y., Xie, J. X., Wang, F. F., Zhong, J., Liu, Y. Z., Li, G. H. & Peng, S. A. (2011). Comparison of ascorbate metabolism in fruits of two citrus species with obvious difference in ascorbate content in pulp. Journal of Plant Physiology, 168, 2196−2205.
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Copyright (c) 2022 Bukola Christiana MAJEKODUNMI, Mariam Opeyemi LOGUNLEKO, Ezekiel Oluwafemi ADEKUNLE, Monsuru Oladimeji ABIOJA, Mojisola Opeyemi ALABA, Abiodun Jelili ADELEKE, Ikechukwu Joseph JAMES, James Olamitibo DARAMOLA
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