INVESTIGATING THE SEROLOGICAL RESPONSE AND SAFETY OF BRUCELLA MELITENSIS REV.1 CONJUNCTIVAL VACCINE IN SMALL RUMINANTS
DOI:
https://doi.org/10.36547/sjas.793Keywords:
Brucella melitensis, conjunctival, mass vaccination, safety, serologyAbstract
Mass vaccination, which is one of the main control policies, provides herd immunity against infectious diseases. This could contribute to the control of the disease and eventually its eradication. The purpose of this study was to investigate the safety and humoral immune response of Brucella melitensis Rev.1 vaccine before the start of mass vaccination. A total of 741 sheep and goats were vaccinated conjunctivally. No adverse effect was observed after the vaccination of the animals. No abortion was seen in pregnant animals. Vaccine strain was isolated from some milk samples taken from only lactating vaccinated goats. Excretion of the vaccine strain was not intense and long-termed. Post-vaccination immune response was evaluated by serological tests, namely, Rose Bengal Plate Test, Serum Agglutination Test and Complement Fixation Test. One month after vaccination, the immune response was high, and the decrease of antibody titers was the highest four to six months after vaccination by inversely correlated with the age of the vaccinated animals. In conclusion, we observed that Brucella melitensis Rev.1 vaccine, used conjunctivally, was safe enough for the animals, and vaccinated animals had high vaccine-induced immune response.
References
Banai, M. (2002). Control of small ruminant brucellosis by use of Brucella melitensis Rev.1 vaccine: laboratory aspects and field observations. Veterinary Microbiology, 90(1−4), 497−519. https://doi.org/10.1016/s0378-1135(02)00231-6
Boschiroli, M.-L., Foulongne, V. & O'Callaghan, D. (2001). Brucellosis: a worldwide zoonosis. Current Opinion in Microbiology, 4(1), 58−64. https://doi.org/10.1016/s1369-5274(00)00165-x
Blasco, J. M. (1997). A review of the use of B. melitensis Rev 1 vaccine in adult sheep and goats. Preventive Veterinary Medicine, 31(3−4), 275−283. https://doi.org/10.1016/s0167-5877(96)01110-5
Briones, G., Iñón de Iannino, N., Roset, M., Vigliocco, A., Paulo, P. S. & Ugalde, R. A. (2001). Brucella abortus Cyclic β-1,2-Glucan Mutants Have Reduced Virulence in Mice and Are Defective in Intracellular Replication in HeLa Cells. Infection and Immunity, 69(7), 4528−4535. https://doi.org/10.1128/iai.69.7.4528-4535.2001
Ducrotoy, M. J., Bertu, W. J., Ocholi, R. A., Gusi, A. M., Bryssinckx, W., Welburn, S. & Moriyón, I. (2014). Brucellosis as an Emerging Threat in Developing Economies: Lessons from Nigeria. PLoS Neglected Tropical Diseases, 8(7), e3008. https://doi.org/10.1371/journal.pntd.0003008
EMEA (2001). The European Agency for the Evaluation of Medicinal Products Committee for Veterinary Medicinal Products 2001. EMEA/CVMP/852/99–FINAL, Note for Guidance Field Trials with Veterinary Vaccines.
Fensterbank, R., Pardon, P. & Marly, J. (1982). Comparison between subcutaneous and conjunctival route of vaccination with Rev.1 strain against Brucella melitensis infection in ewes. Annales de Recherches Veterinaires, 13(4), 295−301.
Fensterbank, R., Pardon, P. & Marly, J. (1985) Vaccination of ewes by a single conjunctival administration of Brucella melitensis Rev.1 vaccine. Annales de Recherches Veterinaires, 16(4), 351−356.
Fensterbank, R., Verger, J. M. & Grayon, M. (1987). Conjunctival vaccination of young goats with Brucella melitensis strain Rev 1. Annales de Recherches Veterinaires, 18(4), 397−403.
Garin-Bastuji, B., Blasco, J. M., Grayon, M. & Verger, J. M. (1998). Brucella melitensis infection in sheep: present and future. Veterinary Research, 29(3-4), 255−274.
Godfroid, J., Cloeckaert, A., Liautard, J. P., Kohler, S., Fretin, D., Walravens, K., Garin-Bastuji, B. & Letesson, J.-J. (2005). From the discovery of the Malta fever's agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. Veterinary Research, 36(3), 313−326. https://doi.org/10.1051/vetres:2005003
Hou, H., Liu, X. & Peng, Q. (2019). The advances in brucellosis vaccines. Vaccine, 37(30), 3981−3988. https://doi.org/10.1016/j.vaccine.2019.05.084
Jiménez de Bagüés, M. P., Marin, C. M., Barberan, M. & Blasco, J. M. (1989) Responses of ewes to B. melitensis Rev1 vaccine administered by subcutaneous or conjunctival routes at different stages of pregnancy. Annales de Recherches Veterinaires, 20(2), 205−213.
Jiménez de Bagüés, M. P., Marín, C. M., Blasco, J. M., Moriyón, I. & Gamazo, C. (1992). An ELISA with Brucella lipopolysaccharide antigen for the diagnosis of B. melitensis infection in sheep and for the evaluation of serological responses following subcutaneous or conjunctival B. melitensis strain Rev.1 vaccination. Veterinary Microbiology, 30(2−3), 233−241. https://doi.org/10.1016/0378-1135(92)90117-c
Lalsiamthara, J. & Lee, J. H. (2017). Development and trial of vaccines against Brucella. Journal of Veterinary Science, 18(S1), 281. https://doi.org/10.4142/jvs.2017.18.s1.281
Minas, A. (2006). Control and eradication of brucellosis in small ruminants. Small Ruminant Research, 62(1−2), 101−107. https://doi.org/10.1016/j.smallrumres.2005.07.031
MFAL (2012). Ministry of Food, Agriculture and Livestock, General Directorate of Food and Control. Control and Eradication Project of Brucella with Conjunctival Vaccine, Circular No: 2012/03.
Nicoletti, P. (2010). Brucellosis: past, present and future. Prilozi, 31(1), 21−32.
O'Callaghan, D. (2020). Human brucellosis: recent advances and future challenges. Infectious Diseases of Poverty, 9, 101. https://doi.org/10.1186/s40249-020-00715-1
OIE. (2018a). World Organisation for Animal Health. Terrestrial Manual. Chapter 3.1.4. Brucellosis (Brucella abortus, B. melitensis, B. suis).
OIE. (2018b). World Organisation for Animal Health Terrrestrial Manual. Chapter 1.1.8 Principles of Veterinary Vaccine Production.
Olsen, S. C. & Stoffregen, W. S. (2005). Essential role of vaccines in brucellosis control and eradication programs for livestock.Expert Review of Vaccines, 4(6), 915−928. https://doi.org/10.1586/14760584.4.6.915
Pappas, G., Papadimitriou, P., Akritidis, N., Christou, L. & Tsianos, E. V. (2006). The new global map of human brucellosis. The Lancet Infectious Diseases, 6(2), 91−99. https://doi.org/10.1016/s1473-3099(06)70382-6
Stournara, A., Minas, A., Bourtzi-Chatzopoulou, E., Stack, J., Koptopoulos, G., Petridou, E. & Sarris, K. (2007). Assessment of serological response of young and adult sheep to conjunctival vaccination with Rev-1 vaccine by fluorescence polarization assay (FPA) and other serological tests for B. melitensis. Veterinary Microbiology, 119(1), 53−64. https://doi.org/10.1016/j.vetmic.2006.08.004
VICH. (2008). International Cooperation on Harmonisation of Technical Requirements for Registration of Veterinary Medicinal Products Guideline, VICH GL 44, Target Animal Safety for Veterinary live and inactivated vaccines.
Wareth, G. (Coord.) (2019). Brucellosis in the Mediterranean countries: history, prevalence, distribution, current situation and attempts at surveillance and control. OIE Technical Series, Volume 12. ISBN 978-92-95115-00-2
Yumuk, Z. & O'Callaghan, D. (2012). Brucellosis in Turkey − an overview. International Journal of Infectious Diseases, 16(4), e228−e235. https://doi.org/10.1016/j.ijid.2011.12.011
Zhang, N., Huang, D., Wu, W., Liu, J., Liang, F., Zhou, B. & Guan, P. (2018). Animal brucellosis control or eradication programs worldwide: A systematic review of experiences and lessons learned. Preventive Veterinary Medicine, 160, 105−115. https://doi.org/10.1016/j.prevetmed.2018.10.002
Zundel, E., Verger, J. M., Grayon, M. & Michel, R. (1992). Conjunctival vaccination of pregnant ewes and goats with Brucella melitensis Rev 1 vaccine: safety and serological responses. Annales de Recherches Veterinaires, 23(2), 177−188.
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Copyright (c) 2023 Sevil Erdenlig Gurbilek, Mustafa Sencer Karagul, Ahmet Murat Saytekin, Emin Ayhan Baklan, Gulnur Saglam
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