SCREENING OF INTERNAL AND EXTERNAL BACTERIA FOUND IN FARMED FISH IN RELATION TO FEED QUALITY

Authors

  • Harrison Ngige Egerton University
  • Mwajuma Abubakar Egerton University
  • Elijah Lelmen Egerton University

DOI:

https://doi.org/10.36547/sjas.872

Keywords:

aquaculture, pathogens, fish feeds, tilapia, catfish, health risks

Abstract

Aquaculture in Kenya is progressively developing as an alternative means of human food production and subsistence. An enormous loss in aquaculture has been accelerated by the mortality of Nile Tilapia (Oreochromis niloticus) and African catfish (Clarias gariepinus) fingerlings. This study aimed at screening the internal and external bacteria found in farmed Nile tilapia and African Catfish at Egerton University agro-science fish farm. The microbiology laboratory collected and processed fifteen healthy fish and fish feeds (formulated and commercial). The skin, gills, and intestines were processed and cultured in Salmonella Shigella and Blood agar. Commercial and formulated fish feeds were also processed and cultured in Salmonella Shigella and Blood agar. The resulting bacteria colonies were subjected to morphological examination and biochemical tests for identification. The findings of this study demonstrated the existence of Enterobacteriaceae. Escherichia, Salmonella, and Bacillus species were the most prevalent species identified on the skin, gills, and intestines. Formulated feeds were more contaminated with bacteria than commercial feeds. The presence of the above microorganisms, some of which are pathogens (Salmonella enteritidis, Enterobacter aerogenes, Klebsiella oxytoca) to humans, is an indication that undercooked fish may pose health risks to susceptible human beings and an impediment to the rapid intensification of aquaculture as a result of mortality of fingerings.

References

Afolabi, O. J., Oladele, O. O., & Olususi, F. C. (2020). Assessment of bacterial loads of Clarias gariepinus (Burchell, 1822) obtained from cultured and natural habitats. The Journal of Basic and Applied Zoology, 81(1). https://doi.org/10.1186/s41936-020-00168-w

Ahmed, O. M. (2019). Bacteria Associated with Fresh-water Aquaculture Tilapia Fish (Oreochromis niloticus) in Suez, Egypt. Food Science & Nutrition Research, 2(1). https://doi.org/10.33425/2641-4295.1009

Bekele, B., Workagegn, K., & Natarajan, P. (2019). Prevalence and Antimicrobial Susceptibility of Pathogenic Bacteria in Nile Tilapia, Oreochromis niloticus L. International Journal of Aquaculture and Fishery Sciences, 5(4), 022–026. https://doi.org/10.17352/2455-8400.000047

Bergey's Manual of Systematic Bacteriology (Volume 2, Parts A−C, 2nd Edition). (2001−2005). Part A: ISBN-10: 0-387-24143-4: ISBN-13: 978-0387-24143-2. Part B: ISBN-10: 0-387-24144-2; ISBN-13: 978-0387-24144-9. Part C: ISBN-10: 0-387-24145-0; ISBN-13: 978-0387- 24145-6. https://doi.org/10.1111/j.1574-695X.2005.00055.x

Charo, F. J., Mbuthia, P. G., Bebora, L. C., & Nguta, J. M. (2022). Occurrence of potential pathogenic and zoonotic bacteria in farmed fish in Machakos and Nyandarua Counties, Kenya. International Journal of Fisheries and Aquatic Studies, 10(6), 112–118. https://doi.org/10.22271/fish.2022.v10.i6b.2755

Chitambo, B., Musso Munyeme, & Hang’ombe, B. M. (2023). Identifying Bacteria with Public Health Significance from Farmed Nile Tilapia (Oreochromis niloticus), Zambia. International Journal of Microbiology, 2023, 1–9. https://doi.org/10.1155/2023/6650378

Clols-Fuentes, J., Nguinkal, J. A., Unger, P., Kreikemeyer, B., & Palm, H. W. (2023). The bacterial community in African catfish (Clarias gariepinus) recirculating aquaculture systems under different stocking densities. Frontiers in Marine Science, 10. https://doi.org/10.3389/fmars.2023.1073250

Debnath, S., McMurtrie, J., Temperton, B., J. Delamare-Deboutteville, Mohan, C. V., & Tyler, C. R. (2023). Tilapia aquaculture, emerging diseases, and the roles of the skin microbiomes in health and disease. Aquaculture International. https://doi.org/10.1007/s10499-023-01117-4

Dissasa, G., Lemma, B. & Mamo, H. (2022). Isolation and identification of major bacteria from three Ethiopian rift valley lakes live and processed fish, and water samples: implications in the sanitary system of fish products. BMC Veterinary Research, 18, 439 (2022). https://doi.org/10.1186/s12917-022-03508-w

Drago, K. C., Kabarozi, R., Majalija, S., Tamale, A., Sengooba, A. & Lubowa Musisi, N. (2016). Isolation and identification of potential probiotic bacteria on surfaces of Oreochromis niloticus and Clarias gariepinus from around Kampala, Uganda. African Journal of Microbiology Research, 10(36), 1524–1530. https://doi.org/10.5897/ajmr2016.8235

Fakorede, C. O., Fatokun, E. N., Blessing Philip Kantiok, Charles Shey Wiysonge, & Ishmael Festus Jaja. (2019). Bacteriological Assessment and Antibiotics Susceptibility Profile of Bacteria Recovered from Pond Water, Fish Skin, and Gut in Ile-Ife, Osun State, Nigeria. https://doi.org/10.20944/preprints201904.0108.v1

Food and Agriculture Organization of the United Nations. (2020a). The State of World Fisheries and Aquaculture 2020. FAO. https://doi.org/10.4060/ca9229en

Jamabo, N. A., Ukwe, I. O. K. & Amachree, D. (2019). Growth Assessment and Microbial Flora Presence in African Catfish (Clarias gariepinus) Larvae Fed Live and Commercial Feeds. International Journal of Sciences, 8(7), 1–6. https://doi.org/10.18483/ ijsci.2058

Karimi, R. D., Ngeranwa, J. J., Njagi, E. N., &Kariuki, S. (2022). The bacterial flora of Oreochromis niloticus and Clarias gariepinus from earthen ponds in Sagana and Masinga, Kenya. International Journal of Bonorowo Wetlands, 12(2). https://doi.org/10.13057/bonorowo/w120202

Kyule-Muendo, D., Otachi, E. O., Awour, F., Erick Ochieng Ogello, Obiero, K. O., Abwao, J., Muthoni, C. N., & Jonathan Mbonge Munguti. (2022). Status of fish health management and biosecurity measures in fish farms, cages, and hatcheries in Western Kenya. CABI Agriculture and Bioscience, 3(1), 18. https://doi.org/10.1186/s43170-022-00086-7

M. Ogbukagu, C., G.Anaukwu, C., C. Ekwealor, C., N Mba, A., & A. Ekwealor, I. (2020). Physicochemical and Antibacterial Susceptibility Profile of Fish Pond Waters in Anambra State, Nigeria. American Journal of Microbiological Research, 8(4), 150–159. https://doi.org/10.12691/ajmr-8-4-5

Mumbo, M. T., Nyaboga, E. N., Kinyua, J., Muge, E. K., Scholastica G. K. Mathenge, Muriira, G., Rotich, H., Njiraini, B., & Njiru, J. M. (2023). Prevalence and antimicrobial resistance profile of bacterial foodborne pathogens in Nile tilapia fish (Oreochromis niloticus) at points of retail sale in Nairobi, Kenya. 2. https://doi.org/10.3389/frabi.2023.1156258

Mzula, A., Wambura, P. N., Mdegela, R. H., & Shirima, G. M. (2020). Present status of aquaculture and the challenge of bacterial diseases in freshwater farmed fish in Tanzania; A call for sustainable strategies. Aquaculture and Fisheries. https://doi.org/10.1016/j.aaf.2020.05.003

Njagi, I. G. (2019). Microbial Contamination of Nile Tilapia (Oreochromis Niloticus) and African Catfish (Clarias Gariepinus) Fingerlings Reared in Hatchery Tanks at Sagana, Jambo, and Mwea Fish Farms. Ku.ac.ke. http://ir-library.ku.ac.ke/handle/123456789/19987

Ogbukagu, C. M., Anaukwu, C. G., Ekwealor, C. C., N. Mba, A., & Ekwealor, I. A. (2021). Bacteriological Assessment and Antibiotic Susceptibility Profile of Bacteria Recovered from Clarias Gariepinus Selected from Various Fish Farms in Anambra North Senatorial Zones in Anambra State, Nigeria. Advances in Microbiology, 11(05), 243–256. https://doi.org/10.4236/aim.2021.115018

Ojwala, R. A., Otachi, E. O., & Kitaka, N. K. (2018). Effect of water quality on the parasite assemblages infecting Nile tilapia in selected fish farms in Nakuru County, Kenya. Parasitology Research, 117(11), 3459–3471. https://doi.org/10.1007/s00436-018-6042-0

Opiyo, M. A., Marijani, E., Muendo, P., Odede, R., Leschen, W., & Charo-Karisa, H. (2018). A review of aquaculture production and health management practices of farmed fish in Kenya. International Journal of Veterinary Science and Medicine, 6(2), 141–148. https://doi.org/10.1016/j.ijvsm.2018.07.001

Ringø, E., Van Doan, H., Lee, S. H., Soltani, M., Hoseinifar, S. H., Harikrishnan, R., & Song, S. K. (2020). Probiotics, lactic acid bacteria, and bacilli: interesting supplementation for aquaculture. Journal of Applied Microbiology, 129(1), 116–136. https://doi.org/10.1111/jam.14628

Tesfaye, S., Kasye, M., Chane, M., Bogale, B., & Abebe agere, Z. (2018). Preliminary Survey of Gram-Negative Bacterial Pathogens from Commonly Caught Fish Species (Oreochromis niloticus, Cyprinus carpio and Clarias gariepinus) in Lake Hayiq, Ethiopia. Fisheries and Aquaculture Journal, 09(01). https://doi.org/10.4172/2150-3508.1000238

Wamala, S. P., Mugimba, K. K., Mutoloki, S., Evensen, Ø., Mdegela, R., Byarugaba, D. K., & Sørum, H. (2018a). Occurrence and antibiotic susceptibility of fish bacteria isolated from Oreochromis niloticus (Nile tilapia) and Clarias gariepinus (African catfish) in Uganda. Fisheries and Aquatic Sciences, 21(1). https://doi.org/10.1186/s41240-017-0080-x

Wanja, D. W., Mbuthia, P. G., Waruiru, R. M., Mwadime, J. M., Bebora, L. C., Nyaga, P. N., & Ngowi, H. A. (2019). Bacteria; pathogens isolated from farmed fish and source pond water in Kirinyaga County, Kenya. International Journal of Fisheries and Aquatic Studies, 7 (2), 295-301.

Wanja, D. W., Mbuthia, P. G., Waruiru, R. M., Mwadime, J. M., Bebora, L. C., Nyaga, P. N. & Ngowi, H. A. (2020). Fish Husbandry Practices and Water Quality in Central Kenya: Potential Risk Factors for Fish Mortality and Infectious Diseases. Veterinary Medicine International, 2020, 1−10. https://doi. org/10.1155/2020/6839354

Zaky, M. M. M., & Ibrahim, M. E. (2017). Screening of Bacterial and Fungal Biota Associated with Oreochromis niloticus in Lake Manzala and Its Impact on Human Health. Health, 09(04), 697–714. https://doi.org/10.4236/health.2017.94050

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Published

2024-06-30

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Reviews