EFFECT OF ENCAPSULATED ROSEMARY EXTRACT ON TURKEY SEMEN BACTERIAL CONTAMINATION DURING SHORT-TERM LOW TEMPERATURE STORAGE
DOI:
https://doi.org/10.36547/sjas.1120Keywords:
Bacterial contaminationAbstract
This study was conducted to define the bacterial community present in turkey semen and evaluate the rosemary essential oil potential in un-encapsulated and encapsulated forms on inhibition of bacterial growth during semen short-term low temperature (4 oC) storage. Semen samples were collected from mature male turkeys and its' bacterial profile was determined through MALDI Biotyper. The semen samples were treated with different concentrations (0, 5, 10 and 25 µg/mL) of un-encapsulated and encapsulated forms of rosemary essential oil. The total bacterial count and sensitivity of various bacterial strains to increasing levels of rosemary extract forms was determined at 0, 2 and 24 h of storage. Rosemary essential oil supplementation significantly reduced the semen bacterial load and most bacterial species showed considerable sensitivity to rosemary essential oil application. The sensitivity of bacterial strains to rosemary extract forms increased in response to extension of storage time and this sensitivity was more profound with encapsulated rosemary extract supplementation. These findings suggest that rosemary essential oil can suppress bacterial growth in turkey semen extenders. Moreover, encapsulation may mitigate the toxicity involved in high essential oil doses and support prolonged inhibition of bacterial growth.
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