Post-thaw characteristics of Pinzgau bull semen following long-term and short-term storage

Authors

  • E. ŠPALEKOVÁ
  • B. KULÍKOVÁ
  • A. BALÁŽI
  • A. MAKAREVICH
  • P. CHRENEK

Keywords:

Pinzgau cattle, sperm, storage, viability

Abstract

The aim of the study was to examine sperm viability of Pinzgau bull insemination doses following long- or short-term storage. Insemination doses, provided by Slovak Biological Services Inc. (Lužianky, Slovak Republic), were slow-frozen and stored in containers with liquid nitrogen for 1 - 18 years. The sperm samples were divided, according to the length of storage, to the short-term ( ≤ 15 years) and long-term ( > 15 years) groups. Post-thaw sperm assessment included total motility (CASA), dead/necrotic sperm occurrence (propidium iodide) and sperm morphology examination. No significant influence of storage length on the spermatozoa characteristics of Pinzgau bulls was noted. However, high inter-male variability in the susceptibility of Pinzgau bull sperm to cryodamages was found (P ≤ 0.05). Our results suggest that bull´ individual variability should be taken into account when semen doses are to be stored as genetic resources for a future use.

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Published

2015-09-30

Issue

Vol. 48 No. 3 (2015)

Section

Articles

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