Evaluation of chromosomal aneuploidy in mouse fibroblasts of two different cell lines CD1 and NiH-3T3

Abstract

The aim of this study was cytogenetic analysis of CD1 and NiH-3T3 mouse embryonic fibroblast cell lines. Numerical chromosome aberrations were detected in chromosome sets at metaphase stage; metaphase arrest was achieved by the use of colcemid at the final concentration 5.10-7 M. Analysis of mouse fibroblast nuclei (90 metaphase plates per cell line) showed 72.22 % occurrence of correct diploid nuclei for CD1 and 62.22 % for NiH-3T3 cell line. Total aneuploidy detected in mouse embryonic fibroblasts was 17.78 % for CD1 in comparison to 27.78 % in NiH-3T3 cell line. According to obtained results, mouse embryonic fibroblasts of NiH-3T3 cell line exhibit higher aneuploidy level compared to CD1 cell line. Results from various types of analysis on different fibroblast cell lines including chromosomal studies may be helpful in selection of appropriate quality fibroblast cell lines for further embryonic stem cell culture.