Genetic polymorphism of leptin gene using PCR-RFLP method in three diferent populations

Authors

  • M. A. AZARI
  • S. HASANI
  • M. HEIDARI
  • S. YOUSEFI

Keywords:

leptin gene, Holstein, Mazandarani native cattle, buffaloes, PCR-RFLP

Abstract

This investigation was conducted to identify polymorphism of the leptin gene in Holstein cows, Mazandarani native cattle and buffaloes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The frequencies of A and B alleles were 0.4 and 0. 6 - in Holstein cows, 0.56 and 0.44 - in Mazandarani native cattle, 0.61 and 0.35 in buffaloes, respectively. The heterozygote genotype (AB) had the highest frequency in all three populations: 0.73, 0.622 and 0.509 in Holstein cows, Mazandarani cattle and buffaloes, respectively. Chi-square test showed that Mazandarani cattle and buffaloes were in HardyWeinberg equilibrium but Holstein cows were not in equilibrium.

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Published

2012-06-30

Issue

Vol. 45 No. 2 (2012)

Section

Articles