Evaluation of κ-casein and growth hormone gene polymorphism in native Dalagh sheep

Authors

  • M. A. AZARI
  • S. YOUSEFI
  • E. DEHNAVI

Keywords:

CSN3, GH, Dalagh sheep, PCR-SSCP

Abstract

In this research for studying genetic polymorphism of κ-casein (CSN3) and growth hormone (GH) genes as candidate genes for marker-assisted selection in different domestic livestock species, blood samples (120 samples) were collected from Dalagh sheep randomly. DNA was extracted from blood samples and a 416 bp fragment from exon 4 of the CSN3 gene and a 365 bp region from exon 5 of the GH gene were amplificated by polymerase chain reaction. PCR products were analyzed using single strand conformation polymorphism (SSCP) method on a polyacrilamide gel. Results indicate that there are two (K1=0.92 and K2=0.08) and three (G1=0.84, G2=0.13 and G3=0.03) conformational patterns for CSN3 and GH genes, respectively.

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Published

2011-12-31

Issue

Vol. 44 No. 4 (2011)

Section

Articles