Influence of different non starch polysaccaride degrading feed enzymes on the intestinal microbiota in piglets

Authors

  • T. OSSWALD
  • W. VAHJEN
  • O. SIMON

Keywords:

piglet, bacteria, NSP, feed enzymes, growth

Abstract

In order to estimate the growth potential of intestinal bacteria under the influence of different types of enzymes, 1,4 â-arabinoxylan from wheat, 1,3 – 1,4 â-glucan from barley or modified wheat extracts were preincubated for 1 hour with an endo 1,4 â-xylanase- or a multienzyme preparation before incubation with intestinal contents of weaned piglets (56d). Compared to controls, both enzyme preparations inhibited bacterial growth in stomach contents in incubations with 1,4-â arabinoxylan as substrate, but the multienzyme preparation led to higher bacterial growth in jejunum and colon contents. A growth increase was noted for the multienzyme preparation and 1,3 – 1,4-â glucan as substrate in all intestinal segments, while the monoenzyme showed no difference with this substrate. A trend for growth reduction for both enzyme preparations in stomach- and in part in jejunum contents was also visible in incubations with a large molecular size wheat extract (> 30 kD). Only minor differences were observed for its low molecular counterpart (< 30 kD) and endo 1,4 â-xylanase preincubation, but the presence of the multienzyme preparation showed increased growth. This in vitro study has shown that intestinal bacteria react differently to the presence of non – starch polysaccharide (NSP) degrading enzymes. Production of multiple NSP fragments by the multienzym preparation from the wheat extracts may enhance bacterial growth in the jejunum, while the hydrolysis of 1,4-â arabinoxylans with the 1,4-â arabinoxylanase seemed to inhibit bacteria in all tested intestinal segments.

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Published

2006-06-30

Issue

Vol. 39 No. 1-2 (2006)

Section

Articles