Effect of subchronic exposure to tolylfluanid on the lactate dehydrogenase activity and its isoenzymes in sheep

Abstract

Tolylfluanid (1,1-dichloro-N-[dimethylamino)-sulfonyl]-1-fluoro-N-(4-methylphenyl) methanesulphenamide is a foliar basic fungicide with protective action and additional acaricidal effects. In this study we have evaluated sub-chronic effects of tolylfluanid, administered at a dose of 93 mg.kg-1 b.wt (1/20 LD50) for 7 days/week for a period of 28, 60 and 90 days on the activities of LDH and its isoenzymes in the blood plasma and haemolyzates of sheep. The data showed no significant differences in total activity of LDH (µkat.l-1) and its isoenzymes (%) in the blood plasma of the control and experimental animals examined before the experiment and after 28-, 60- and 90-day-exposure. In the experimental group of sheep significant changes of LDH-1 isoenzyme (P=0.046) and LDH-2 isoenzyme (P=0.011) activities in haemolyzates after 28- and 90-day exposure to tolylfluanid have been registered.