RABBIT ADIPOSE-DERIVED STEM CELLS MAINTAIN THEIR CHROMOSOMAL COUNTS DURING PASSAGING

Authors

  • Mária Tomková Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Nitra, Slovak Republic
  • Jaromír Vašíček Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Nitra, Slovak Republic; NPPC – Research Institute for Animal Production Nitra, Lužianky, Slovak Republic
  • Andrej Baláži NPPC – Research Institute for Animal Production Nitra, Lužianky, Slovak Republic
  • Jozef Čurlej Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Nitra, Slovak Republic
  • Peter Chrenek Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, Nitra, Slovak Republic; NPPC – Research Institute for Animal Production Nitra, Lužianky, Slovak Republic

Keywords:

rabbit, adipose derived stem cells, chromosomes, karyotype

Abstract

Monitoring of stem cells genetic stability is one of the most important safety points, because the number of chromosomes can change throughout the culture and in vitro manipulation of these cells. In our study the stem cells metaphases were analysed using the G-staining method. At least 60 metaphases in three samples of rabbit adipose-derived stem cells were assessed in three subsequent passages. Results of our study showed that at least 70 % of cells in each passage can maintain their stable karyotype. The highest proportion of aneuploidy (30 %) was recorded in the third passage. Even though we observed a slight increase of aneuploidy during passaging, statistical analysis did not show any significant differences. Based on our results, we can conclude that cell passaging does not affect genetic stability, since there were no changes in chromosomal counts throughout the culture. However, it was observed that there may be some instabilities during passaging that are more random. For this reason, it is recommended to monitor the stem cells' karyotype, especially if they are intended for therapeutic use.

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Published

2019-12-16

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Short Communication

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