SPERM MOTILITY OF RAMS FROM TWO SLOVAK SHEEP BREEDS: SHORT COMMUNICATION
Keywords:
slovak rams, sperm, quality, motility, morphologyAbstract
In the present study, fresh ram sperm samples (n = 52) from the Native Wallachian (NW; n = 26) and Improved Wallachian (IW; n = 26) Slovak sheep breeds were collected from males of each breed by electro-ejaculation and analysed for motility using CASA and morphology using light microscopy assessment. Our results showed no significant differences between the breeds (P ≤ 0.05) in the sperm concentration and motility traits (Table 1). Also both these ram groups did not differ (P ≤ 0.05) in sperm morphology parameters (Table 2). Since, this is only preliminary research, additional experiments using higher number of samples (rams) as well as other evaluation approaches are required in order to compare sperm characteristics for different breeds.
References
AX, R. L., DALLY, M., DIDION, B. A., LENZ, R. W., LOVE, C. C., VARNER, D. D., HAFEZ, B. & BELLIN, M. E. (2016). Asisted Reproductive Technology: Semen Evaluation. Reproduction of Farm Animals. Philadelphia: Lippincott Williams & Wilkins, p. 363–375. ISBN 978-11-192-6530-6.
DAVID, I., ROBERT-GRANIÉ, C., MANFREDI, E., LAGRIFFOUL, G. & BODIN, L. (2008). Environmental and genetic variation factors of artificial insemination success in French dairy sheep. Animal, 2, 979–986.
CHRENEK, P., MAKAREVICH, A. V., KUBOVICOVA, E., BULLA, J. & SUPUKA, P. (2019). Slovak National Animal Breeds. Nitra: Slovak University of Agriculture. p. 16–20. ISBN 978-80-552-1982-0.
KUBOVICOVA, E., MAKAREVICH, A. V., SPALEKOVA, E. & HEGEDUSOVA, Z. (2011). Motility and fertilizing ability of frozen-thawed ram sperm from two sheep breeds. Slovak Journal of Animal Science, 2011, 44(4), 134–139.
KULIKOVA, B., BALAZI, A., TOTHOVA, J., JURCIK, R.,HUBA, J. & CHRENEK, P. (2018). Dilution factor affects the ability of ram sperm to survive cryopreservation: Short communication. Slovak Journal of Animal Science, 51(1), 41–44.
KULAKSIZ, R., CEBI, C. & AKCAY, E. (2012). The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. The Turkish Journal of Veterinary and Animal Sciences, 36(2), 177–182.
MAROTO-MORALES, A., RAMON, M., GARCIA-ALVAREZ, O., SOLER, A. J., ESTESTO, M. C., MARTINEZ-PASTOR, F., PEREZ-GUZMAN, M. D. & GARDE, J. J. (2010). Characterization of ram (Ovis aries) sperm head morphometry using the sperm-class analyzer. Theriogenology, 73(4), 437–448.
O'HARA, L., HANRAHAN, J. P., RICHARDSON, L., DONOVAN, A., FAIR, S., EVANS, A. C. O. & LONERGAN, P. (2010). Effect of storage duration, storage temperature, and diluent on the viability and fertility of fresh ram sperm. Theriogenology, 73(4), 541–549.
