Viability assessment of fresh chicken blastodermal cells by flow cytometry

Authors

  • A. SVORADOVÁ
  • L. KUŽELOVÁ
  • J. VAŠÍČEK
  • P. CHRENEK

Keywords:

chicken, blastodermal cells, viability, flow cytometry

Abstract

This study describes the viability assessment of fresh chicken blastodermal cells (BCs) by flow cytometry using a novel DNAdetecting far-red-fluorescing dye, DRAQ5 and LIVE/DEAD (LD) Fixable Green Dead Cell Stain Kit. Overall, the technique provides both quantitative and qualitative evaluation of BCs. Particular attention was put on the determination of a portion of nucleated (DRAQ5+ ) and dead (DRAQ5+ /LD+ ) BCs from freshly isolated samples. The results showed that the proportion of nucleated cells from a sample was ranged from 50.39 to 72.58 % and dead cells from 0.40 to 11.38 % in all repetitions. Furthermore, our study suggests that flow cytometry allows precise examination of fresh BCs viability in a very short time and that a combination of DRAQ5/LD staining is suitable for the detection of nuclear BCs and their viability evaluation, respectively. Moreover, this technique might also be applied for the evaluation of frozen-thawed BCs after cryopreservation process for animal gene bank.

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Published

2018-06-30

Issue

Vol. 50 No. 2 (2017)

Section

Articles

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