Genetic analyse of selected breeds of gooses bred in Slovakia

Abstract

Our work describes the results of electrophoretic separation of seven polymorphic proteins, which were analysed in hemolysate and blood plasma of three breeds of gooses bred in Slovakia – Suchovska goose (n = 74), Slovak White goose (n = 34) and Teshedikovska goose (n = 148). The results of the analyses were divided into two groups according to number of alelles of biochemical polymorphic markers: the 1st group – monomorphic variant A for hemoglobin (HbA); the 2nd group – two-alelles polymorphism for the loci esterase 1 (Es1A, Es1B), carbonic anhydrase (CaF , CaS ), esterase 3 (Es3A, Es3B), transferin (TfA, TfB), alkalic phosphatase (AfA, AfB) and ceruloplasmin (CpA, CpB). The genotypic structures - AA, AB and BB were identified in genetic markers of blood -plasma and erythrocytes (enzymes and proteins) Es1, Es3, Tf, Af and Cp. Genotypes of carbonic anhydrase were described as FF, FS and SS as per the convetion. According to a locus of hemoglobin, only a monomorphic genotypic combination of AA was observed. Statistical analyse was carried out using the statisical formula for two-alelle polymorphic system. Obtained alellic frequencies in selected coding loci were used for computing genetic distances (D) among tested breeds of geese by means of statictical programme PHYLIP ver. 3.63 (http://www.phylip.com). The lowest value of gegetic distance was found between Slovak White goose and Suchovska goose (D = 0,01834) and the highest value (D = 0,08334) was recorded between Suchovska and Tesedikovska geese.