THE USE OF HOMA-IR AND QUICKI IN RODENT DIABETIC MODEL: SHORT COMMUNICATION
Keywords:
HOMA-IR, QUICKI, diabetes, Zucker diabetic fatty rats, dietAbstract
Regarding diabetes mellitus type 2 (DMT2) in animal research, a non-invasive and less traumatic method, such as the mathematic calculation of indexes expressing the insulin sensitivity and resistance, is required. There are some methods and formulas for calculation and estimation of insulin resistance. The most well-known validated methods are the homeostatic model assessment of insulin resistance (HOMA-IR) and the quantitative insulin sensitivity check index (QUICKI), which are suitable for clinical and research purposes. The goal of this study was to calculate HOMA-IR and QUICKI indexes in an experiment with Zucker diabetic fatty (ZDF) rats fed normal or high-energy diet. Additionally, the correlations between both models were inquired. Animals were divided into three groups: lean untreated control rats (C, n = 10) fed a complete feed mixture for rats and mouse (10 MJ.kg-1), diabetic rats fed the same chow (E1, 10 MJ.kg-1) and diabetic rats fed high energy diet (E2, enriched KKZ-P/M, 20 MJ.kg-1). After overnight fasting, the rats were monitored for blood glucose level by a FreeStyle Optium Neo Glucose and Ketone Monitoring System (Abbott Diabetes Care Ltd., UK) using test strips. An ELISA commercial kit (Biotech, Bratislava, Slovak Republic) was used to measure the serum content of insulin. Values of fasting plasma insulin and serum glucose were used to calculate HOMA-IR and QUICKI indexes. HOMA-IR and QUICKI significantly differed among the groups. Strong negative correlations were found in dependence on the diet. This study indicated that the calculation of HOMA-IR and QUICKI can potentially be an effective tool in determination, evaluation, onset and progress of DMT2.
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